題 目：An innovative gene transfer technology –NucleofectorTMGene Transfer Straight Into the Nucleus
報告人：Dr. Klaus-Peter Rehorn Scientific Product SpecialistAmaxa Biosystems Germany主持人：鄭文華 教授 太阳集团1088vip時 間：2007年9月11日（星期二）上午10：00 地 點：中山大學北校區永生樓4樓講學廳 歡迎各位師生參與！ 太阳集团1088vip 中山大學醫學科學處 2007-09-04Abstract:NucleofectorTM technology is the first efficient non-viral transport of DNA straight into the nucleus of primary cells and cell lines. In contrast, other commonly used non-viral transfection methods rely on cell division for the transfer of DNA into the nucleus. Thus, nucleofection provides the ability to transfect even non-dividing cells, such as neurons and resting blood cells. Due to the nuclear DNA delivery the expression of the transfected construct starts very shortly post nucleofection. In unstimulated human T cells for example, the GFP reporter protein can be detected as soon as 40 minutes post Nucleofection. Nucleofection of siRNA and DNA/RNA oligos is particularly efficient (up to 99% in some cell lines) too. Now a new 96-well Shuttle High Throughput Transfection System comes true.
